pcxwb ebna1 (Addgene inc)
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pcxwb ebna1
Pcxwb Ebna1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcxwb+ebna1/pCXWB-EBNA1+(Plasmid+%2337624)/pm41760600-332-59-60
Average 95 stars, based on 85 article reviews
Pcxwb Ebna1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcxwb+ebna1/pCXWB-EBNA1+(Plasmid+%2337624)/pm41760600-332-59-60
Average 95 stars, based on 85 article reviews
pcxwb ebna1 - by Bioz Stars,
2026-09
95/100 stars
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Cell Culture:Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration. Article Snippet: .. Briefly, cryopreserved PBMCs (5 × 106 cells) were thawed and cultured for six days in StemSpanTM using the Human CD34+ Cell NucleofectorTM Kit (Lonza, Cat. #VPA-1003) with the following episomal plasmids (2 g each): pCXLE-hOCT3/4- shp53-F (Addgene, Cat. #27077; encoding OCT3/4 and TP53 shRNA), pCXLE-hUL (Addgene, Cat. #27078; L-MYC and LIN28), pCXLE-hSK (Addgene, Cat. #27080; SOX2 and KLF4) and Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration Article Snippet: .. Briefly, cryopreserved PBMCs (5 × 10 6 cells) were thawed and cultured for six days in StemSpanTM using the Human CD34 + Cell NucleofectorTM Kit (Lonza, Cat. #VPA-1003) with the following episomal plasmids (2 μg each): pCXLE-hOCT3/4-shp53-F (Addgene, Cat. #27077; encoding OCT3/4 and TP53 shRNA), pCXLE-hUL (Addgene, Cat. #27078; L-MYC and LIN28), pCXLE-hSK (Addgene, Cat. #27080; SOX2 and KLF4) and shRNA:Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration. Article Snippet: .. Briefly, cryopreserved PBMCs (5 × 106 cells) were thawed and cultured for six days in StemSpanTM using the Human CD34+ Cell NucleofectorTM Kit (Lonza, Cat. #VPA-1003) with the following episomal plasmids (2 g each): pCXLE-hOCT3/4- shp53-F (Addgene, Cat. #27077; encoding OCT3/4 and TP53 shRNA), pCXLE-hUL (Addgene, Cat. #27078; L-MYC and LIN28), pCXLE-hSK (Addgene, Cat. #27080; SOX2 and KLF4) and Article Title: STING activation induces polarized cytokine secretion of IFN-β and IL-17A promoting photoreceptor death and choroidal disruption in age-related macular degeneration Article Snippet: .. Briefly, cryopreserved PBMCs (5 × 10 6 cells) were thawed and cultured for six days in StemSpanTM using the Human CD34 + Cell NucleofectorTM Kit (Lonza, Cat. #VPA-1003) with the following episomal plasmids (2 μg each): pCXLE-hOCT3/4-shp53-F (Addgene, Cat. #27077; encoding OCT3/4 and TP53 shRNA), pCXLE-hUL (Addgene, Cat. #27078; L-MYC and LIN28), pCXLE-hSK (Addgene, Cat. #27080; SOX2 and KLF4) and Transfection:Article Title: Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor. Article Snippet: Ficoll-PaqueTM PREMIUM solution (Stem Cell Technologies) was used for isolating mononuclear cells from a donor’s blood following described method. .. The separated mononuclear cells, 2x105 PMBCs, were washed with PBS before transfected with episomal plasmid vectors (0.83 μg/μl of pCXLE-hOCT3/4-shp53-F (Addgene Plasmid #27077), 0.83 μg/μl of pCXLE-hSK (Addgene Plasmid #27078), 0.83 μg/μl of pCXLE-hUL (Addgene Plasmid #27080) and 0.5 μg/μl of Article Title: Generation of induced pluripotent stem cell lines (RFSCi003-A, RFSCi004-A) from monozygotic twins discordant for age-related macular degeneration. Article Snippet: After 6 days of expansion, cells were harvested for nucleofection using the Human CD34 Cell NucleofectorTM Kit (Lonza, Cat. # VPA-1003) with program U-008. .. The transfection mixture contained the following episomal plasmids (2 μg each): pCXLE-hOCT3/4shp53 (Addgene, Cat # 27077), pCXLE-hUL (Addgene, Cat # 27078; expressing L-MYC/LIN28), pCXLE-hSK (Addgene, Cat # 27080; expressing SOX2/KLF4), Plasmid Preparation:Article Title: Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor. Article Snippet: Ficoll-PaqueTM PREMIUM solution (Stem Cell Technologies) was used for isolating mononuclear cells from a donor’s blood following described method. .. The separated mononuclear cells, 2x105 PMBCs, were washed with PBS before transfected with episomal plasmid vectors (0.83 μg/μl of pCXLE-hOCT3/4-shp53-F (Addgene Plasmid #27077), 0.83 μg/μl of pCXLE-hSK (Addgene Plasmid #27078), 0.83 μg/μl of pCXLE-hUL (Addgene Plasmid #27080) and 0.5 μg/μl of Article Title: Lamin B1 physically regulates neuronal migration by modulating nuclear deformability in the developing cortex Article Snippet: .. A Y4 episomal plasmid mixture was prepared, consisting of pCXLE-hOCT3/4-shp53-F (Addgene, Cat# 27077), pCXLE-hSK (Addgene, Cat# 27078), pCXLE-hUL (Addgene, Cat# 27080), Construct:Article Title: Lamin B1 physically regulates neuronal migration by modulating nuclear deformability in the developing cortex Article Snippet: .. The following plasmids were constructed in previous studies: pCAGGS ( ); pCAG-EGFP-N1, pCAG-EGFP-C1, pCAG-H2B-TagRFP ( ); pCXLE- hOCT3/4 - shp53 -F (Addgene, Cat# 27077); pCXLE-hSK (Addgene, Cat# 27078); pCXLE-hUL (Addgene, Cat# 27080); Expressing:Article Title: Generation of induced pluripotent stem cell lines (RFSCi003-A, RFSCi004-A) from monozygotic twins discordant for age-related macular degeneration. Article Snippet: After 6 days of expansion, cells were harvested for nucleofection using the Human CD34 Cell NucleofectorTM Kit (Lonza, Cat. # VPA-1003) with program U-008. .. The transfection mixture contained the following episomal plasmids (2 μg each): pCXLE-hOCT3/4shp53 (Addgene, Cat # 27077), pCXLE-hUL (Addgene, Cat # 27078; expressing L-MYC/LIN28), pCXLE-hSK (Addgene, Cat # 27080; expressing SOX2/KLF4), |